A study of phytochemical screening and antibacterial effect of some methanolic plant extracts on Pseudomonas aeruginosa

Document Type : Research articles.

Authors

1 Botany Department, Faculty of Science, Fayoum University

2 Zoology Department, Faculty of Science, Fayoum University

Abstract

Resistance of the different bacterial strains to conventional antibiotics was noticed, and the need of solutions to come over this problem turn into find natural sources. The efficacy of methanol extracts of different plants is noticed as a powerful way to solve this problem. Lantana camara, Eucalyptus sp, Menthab sp, Mangifera indicia leaves were chosen as trails. Efficacy of methanol extracts of Lantana, Eucalyptus, Mentha, Mangifera leaves were assessed against pathogenic multi-drug resistant Pseudomonas aeruginosa. The resistance of the bacterial strain was screened for all used conventional antibiotics.  The extracts from the investigated leaves have been studied for their antimicrobial and antioxidant effects. The disc diffusion method was used for determining the minimum inhibitory concentration (MIC) and the antimicrobial activity. The same MIC (12.5 mg/mL) was recorded for Eucalyptus and Mentha, while MICs were 3.125, 6.25 mg/mL for Lantana and Mangifera, respectively. Chemical composition of each extract was evaluated using GC-MS giving; hexadecanol as the major compound (15.13%) for Lantana, eucalyptol was identified as the major compound (21.41%) for Eucalyptus, 2-methyl-5-phenyl Cyclohexen-1-one was identified as the main compound (14.31%) for Mentha, and chloroform was identified as the major compound (7.67%) for Mangifera. The total phenols, DPPH scavenging activity, and reducing power were estimated under spectrophotometric conditions and supported our GC-MS findings. In this article, different plant leaves extracts act as powerful antibacterial agents, and the MICs values were significantly low against the tested bacterial pathogen.  

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