ANTIBACTERIAL ACTIVITY OF FUROQUINOLONE ALKALOIDS AGAINST POTATO SOFT ROT BACTERIUM, Erwinia carotovora FROM Ruta chalepensis LEAVES

Emam, Ahmed M.; Mahmoud, Mohamed E.

doi:10.21608/fjard.2005.197845

ANTIBACTERIAL ACTIVITY OF FUROQUINOLONE ALKALOIDS AGAINST POTATO SOFT ROT BACTERIUM, Erwinia carotovora FROM Ruta chalepensis LEAVES

Document Type : Research articles.

Authors

Ahmed M. Emam ¹
Mohamed E. Mahmoud ²

¹ Biochemistry Department, Faculty of Agriculture, Fayoum Branch, Cairo Univ., Egypt.

² Botany Department (Microbiology), Faculty of Science, Cairo Univ., Giza, Egypt.

10.21608/fjard.2005.197845

Abstract

Soft rot bacterium; Erwinia carotovora is one of the most
severe post-harvest diseases of potatoes worldwide. This bacterium
affects tubers during storage, transit and marketing. The aqueous
ethanolic extract (80%) of Ruta chalepensis leaves (Rutaceae)
showed antibacterial activity against Erwinia carotovora (MIC =
625 µg/ml). Bioassay-guided isolation of this aqueous extract by
using a combination of different chromatographic methods (TLC
and column chromatography) yielded two furoquinolone alkaloids
for the first time from this plant. Their structures were
characterized as 2, (hydroxy isopropyl) – 6, hydroxy –9, methyldihydrofuro [2,3] –quinol-4-one (I) and 3, (hydroxy methyl)-6,
methoxy-2, 2, 9-trimethyl-dihydrofuro [2,3] quinol-4-one (II) by
1
H, 13C NMR and MS spectral data. The isolated alkaloids I and II
showed antibacterial activity against Erwinia carotovora with MIC
at 20 and 35 µg/ml respectively.

Keywords