ISOLATION AND IDENTIFICATION OF BACTERICIDE AND MITICIDE COMPOUND FROM SCHINUS TEREBINTHIFOLIUS LEAVES AGAINST THE POTATO ROT BACTERIA AND SPIDER MITE

Document Type : Research articles.

Authors

1 Biochemistry Department, Faculty of Agriculture, Cairo University, Fayoum Branch, Egypt.

2 Plant Protection Department, Faculty of Agriculture, Cairo University, Fayoum Branch, Egypt.

Abstract

The MeOH extracts of 26 plant species belonging to 18 plant
families were examined against the two spotted spider mite
Tetranychus urticae and the two pathogenic, brown rot bacterium
Ralstonia solanacearum and soft rot bacterium Erwinia
carotovora
. The results indicated that Schinus terebinthifolius was
the most potent plant for controlling these pests as its methanol
extract exerted highly activity against the two pathogenic bacteria
in addition to moderate activity against the spider mite. The MeOH
extract of this plant was purified by using a combination of
different chromatographic methods (column chromatography and
TLC) to yield an active pure compound. Based on spectroscopic
methods (
1H,13C-NMR,UV and MS) the isolated compound was
characterized as gallic acid methyl ester, which exerted a miticidal
activity against the two spotted spider mite
Tetranychus urticae
(LC50=58 mg1-1) and antibacterial activity against Erwinia
carotovora
and Ralstonia solanacearum (MLC= 250 and
500µg/ml), respectively.


Keywords