PROPAGATION OF SOME HARD–TO–ROOT ORNAMENTAL PLANTS BY TISSUE CULTURE II – ESTABLISHMENT AND MULTIPLICATION

Hashem, Mahmoud El Sayed; Saadawy, Faisal Mohamed; Gomaa, Salah AbdelAziz; Abdul-Moneem, Nermeen El–Sayed

doi:10.21608/fjard.2005.197791

PROPAGATION OF SOME HARD–TO–ROOT ORNAMENTAL PLANTS BY TISSUE CULTURE II – ESTABLISHMENT AND MULTIPLICATION

Document Type : Research articles.

Authors

¹ Dept. of Horticulture, Faculty of Agriculture, Ain Shams University, Shoubra ElKhema, Cairo, Egypt.

² Dept. of Ornamental Plant Researches, Horticulture Research Institute, Agriculture Research Center, Giza, Egypt.

10.21608/fjard.2005.197791

Abstract

Applying benzyladenine (BA) at 1 or 2 ppm to the culture
medium at the establishment stage, proved to have a significant effect
in increasing survival percentage of Cupressus macrocarpa explants.
On the other hand, survival percentage of Conocarpus explants was
not significantly influenced by the presence or absence of BA. The
use of BA resulted also in the highest multiplication rates of both
Cupressus macrocarpa and Conocarpus erectus, though the effect
was insignificant with the first species. Leaving a heel on the explant
of Conocarpus erectus showed significantly a superior effect in
increasing shoot number, shoot fresh weight and leaf number.
At the multiplication stage, the highest number of shoots
resulted by using BA at 2 and 4 ppm for Cupressus macrocarpa and
Conocarpus erectus, respectively. The highest contents of total
chlorophyll and carotenoids of Cupressus macrocarpa shoots were
those induced by applying kinetin at 6 ppm. Application of BA at 6
ppm to the culture medium was associated with a greater increase in
these contents in the case of Conocarpus erectus.

Keywords