BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF AN EGYPTIAN ISOLATE OF SOYBEAN MOSAIC VIRUS IN EGYPT.

El-Tahlawey, Mohga M.; Amer, M. A.; Mandour, A.

doi:10.21608/fjard.2006.197566

BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF AN EGYPTIAN ISOLATE OF SOYBEAN MOSAIC VIRUS IN EGYPT.

Document Type : Research articles.

Authors

Plant Virology Research Department, Plant Pathology Research Institute, A.R. C., Giza-Egypt.

10.21608/fjard.2006.197566

Abstract

Soybean mosaic Potyviridae (SbMV) was isolated from naturally
infected soybean plants cvs. Giza 111 and Giza 35 grown at Giza
Research Station Farm, Agricultural Research Centre, Giza. The
inoculated indicator plants gave severe mosaic on soybean cv.Giza 111,
mosaic and malformation on cv Giza 35, mild mosaic on cv. Giza 22,
yellow mild mosaic on cv. Giza 83, vein clearing and mild mosaic on
Phaseolus vulgaris cvs Contender. Chlorotic local lesion on
Chenopodium quinoa. The virus was transmitted in non-persistent
manner by aphids. Electron Microscopy showed aggregates of flexuous
virus particles, degenerated mitochondria and inclusions appeared as
pinwheel in cytoplasm of infected soybean leaves. Dot blot
immunoassay (DBIA) and indirect enzyme linked immunosorbent assays
(ELISA) were used as a serological method for detection and
confirmation of SbMV isolate in infected tissues.A reverse transcription–
polymerase chain reaction (RT-PCR) method was used to detect of local
isolate of SbMV in infected plants using specific oligonucleotides
primer. A major RT-PCR fragment (~340 bp) from the coat protein gene
of the SbMV genome was detected.

Keywords