IMPROVEMENT OF BALADY MANDARIN (Citrus reticulate Blanco) THROUGH SELECTION, EVALUATION AND TISSUE CULTURE TECHNIQUE.

Document Type : Research articles.

Authors

Dept. of Horticulture, Fac. of Agric., Fayoum Univ., Egypt.

Abstract

This work was undertaken during the years 2003, 2004, 2005,
2006 and 2007 for improving the productivity of Balady mandarin
(Citrus reticulate Blanco). Firstly, selection and evaluation of some
mandarin seedlings trees were conducted based on yield (fruit quantity
and fruit quality); such trees would be considered as mother trees.
Secondly, after selection and evaluation, mass production of the
selected superior genetic materials (individual trees) was propagated
by means of tissue culture technique as it is rapid clonal propagation
method to rescue the lose of such limited selected source. The results
could be summarized as follows:
I-Selection and evaluation of some superior variants of mandarin
included; number of fruits/tree, yield/tree (kg), fruit weight(g), fruit
volume (cc), peel thickness (cm), number of seeds/fruit, T.S.S.%,
acidity % and vit. C (mg/100ml. juice) content. The results showed that
some promising mandarin trees with high yield and good fruit quality
which could help in improving mandarin productivity in Fayoum
Governorate, Egypt, were detected.
II- Mass production using shoot tip technique. MS basal medium 1962
at full strength supplemented by 1.5 mg/L BA was sufficient for the
best shooting proliferation of mandarin. MS basal medium plus 1.5
mg/L NAA significant increased the rooting percentage, number of
roots and root length of citrus species
III- Anatomical studies show that MS (Murashinge Skoog Basal
Medium) supplemented with 1.5 mg/L NAA enhanced adventitious
roots formed from callus and cambium (i.e. high rooting percentage),
in comparison to the low concentration 0.5 – 1.0 mg/L which
adventitious roots were formed only from cambial zone (low rooting
percentage).

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