BROCCOLI GROWTH, ANTIOXIDANT ACTIVITY AND HEALTH TISSUES RESPONSES TO ASCORBIC ACID UNDER DEFICIT IRRIGATION

Document Type : Research articles.

Authors

1 Horticulture Department, Faculty of Agriculture, Fayoum University, 63514 Fayoum, Egypt

2 Soils and Water Department (Agric. Eng.), Faculty of Agriculture, Fayoum University, 63514 Fayoum, Egypt

Abstract

Two field experiments were conducted during 2013/2014 and
2014/2015 in a private Farm, Ibshway district, Fayoum, Egypt to study deficit
irrigation (60, 80 and 100% ETc) and ascorbic acid foliar application (0, 200
and 400 mg l
-1) on growth, relative water content (RWC), membrane stability
index (MSI), electrolyte leakage (EL), leaf photosynthetic pigments (LPS),
osmoprotectants (OS) and DPPH radical-scavenging activity of broccoli
plants. The obtained results clarified that, irrigation amount at 80% or 100%
ETc, significantly, resulted in higher mean values of stem length and
diameter, number of leaves plant
-1, leaf MSI, RWC, carotenoids, DPPHradical-scavenging and total free amino acids content than irrigation amount
at 60% ETc. On other side, irrigation amounts, irrespective the level used, did
not reflect any noticeable impact on number of branches plant
-1 and head
contents of ascorbic acid. Increasing irrigation water from 60% up to 100%
ETc decreased the contents of leaf EL, chlorophyll A, B, A + B, anthocyanin,
free proline and TSS. Exogenous application of ascorbic acid at
concentrations of 200 and 400 mg l
-1, significantly improved most studied
growth parameters, RWC, MSI, anthocyanin, DPPH, total free amino acids
and endogenous ascorbic acid however, leaf EL decreased gradually.
Moreover, the impact of ascorbic acid, irrespective of the concentration used,
on leaf photosynthetic pigment, free proline and total soluble sugars contents
was at par.


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