ANTIOXIDANT, ANTICANCER AND IMMUNOCYTOCHEMISTRY ACTIVITIES OF THREE SYNTHETIC COUMARIN DERIVATIVES

Document Type : Research articles.

Authors

1 Biochemistry Department, Faculty of Agriculture, Fayoum University, Egypt.

2 Chemistry Department, Morgan State university, USA

3 Animal Health Research Institute, Agricultural Research Center, Egypt.

4 Chemistry Department, Western Michigan University, USA.

Abstract

Many biological studies have indicated that coumarin and its
derivatives can act as chemoperventive agents. In this study, three synthetic
coumarin derivatives were investigated for their free radical scavenging,
anticancer and cell signaling activities. Compound A showed antioxidant
effects with 100% decoloration of DPPH at concentration of more than 150
µg/ml with IC50 of 102.12 ± 0.15 µg/ml. Compound B and C induced
antioxidant effects with 100% decoloration at 100 and 50 µg/ml,
respectively. Also, the IC50 of compounds B and C was 28.35 ± 0.04 and
6.12 ± 0.05 µg/ml, respectively. Moreover, these compounds suppressed
PC-3 prostate and MDA-MB-453 breast cancer cells proliferation with IC50
of 20 µM, 70 µM and 50 µM, respectively for PC-3 cells. However, it needs
higher dosage to inhibit MDA-MB-453 breast cancer cell growth with IC50
at 25 µM, 137 µM and 100 µM, respectively. All of these compounds
activated the apoptotic death signals downstream by activating caspase
pathway. And showed many apoptotic characterizations including, DNA
fragmentation and apoptotic bodies formation.

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