SCREENING OF SOME YEAST ISOLATES FOR THEIR ANTIBACTERIAL ACTIVITY

Document Type : Research articles.

Authors

1 Agricultural Microbiology Department, Faculty of Agriculture, Fayoum University

2 Dairy Research Department, Food Technology Research Institute, Agricultural Research Center

Abstract

Sixty-nine yeast isolates were collected from different sources.
The isolates were tested using agar well diffusion and disc diffusion
assays against eight medically important indicators. Results indicated
that 48 (69.6%) of isolates exhibited antibacterial activity against one or
more of the eight indicator bacteria. When pH was adjusted to 6.5, only
26 isolates were effective. The stability of yeast isolates supernatants at
pH 6.5 against proteolytic enzymes was also tested. Only sixteen isolates
were effective against test microorganisms. When the supernatants were
treated with catalase and trypsin, only thirteen isolates showed
antibacterial activity. The isolate Eg-Y2 was very effective against Cl.
tyrobutyricum which is considered as one of the most dangerous
anaerobic pathogens. . The 13 isolates were identified as C. pelliculosa
,C. guillermondii, C. glabrata, C. famata, Cryptococcus neoformans,
Rodo muciluginosa using the 20 CUX API system.

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Fayoum Journal of Agricultural Research and Development
Volume 32, Issue 1
January 2018
Pages 31-44

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