A SIMPLE, RAPID, EFFICIENT AND LOW COST METHOD FOR MINIPREP DNA FROM DIFFERENT SOURCES

Document Type : Research articles.

Authors

Department of Genetics, Faculty of Agriculture, Fayoum University, Fayoum

Abstract

DNA extraction and purification are routine processes in most
plant genetic transformation laboratories. Although there are different
commercial kits that allow accurate DNA purification, the total cost of
buying multiple sets of these kits can be spectacular. We use spin
column and laboratory solutions to develop a simple method of DNA
purification that can meet different research needs. This method is used
to extract DNA from the leaves of Brassica and genetically modified
plants and also bacteria; extract plasmid DNA from E. coli or
Agrobacterium tumefaciens; purify the DNA fragments of PCR
products and the resulting fragments of digestion with restriction
endonuclease. DNA concentration of optical density (OD) value was
calculated at 260 nm wavelength and OD260 / OD280 ratios were used
to determine DNA quality. The quantity and quality of DNA obtained
by this method was similar to that of isolated DNA using commercial
Kits. In comparison, it has been shown that this method allows
obtaining DNA from different sources with similar quantity and purity
and low costs.

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