Purification and some properties of fungal Xylanase

Abdul Aziz, Marwa; Abu Al Qasim, Nabil; Mansour, Saeed; Abdul Al-Alim, Yasser

doi:10.21608/fjard.2021.188562

Purification and some properties of fungal Xylanase

Document Type : Research articles.

Authors

¹ Microbiology, Soil, Water and Environment Res. Institute, Agric, Res. Center, Giza, Egypt,

² Botany Department, Faculty of Science, Fayoum University, Fayoum, 63514, Egypt

³ Microbiology Department, Faculty of Agriculture, Fayoum University, Fayoum,63514, Egypt

10.21608/fjard.2021.188562

Abstract

Partial purification of xylanase produced by Aspergillus niger strain AUMC 14230 is carried out with 80% saturation of ammonium sulfate under pH5.0 as first step. The second step in partial purification is Acetone 2.5/1 (v/v). The last step is gill filtrations which give the highest specific from both enzymes. Many factors affecting on purified enzymes. The best activity of xylanase was produced till 72 hrs and decreased by (8.8% & 10.9%), respectively after 72 hrs. Xylan (1mg) concentration was the best substrate to produce relative activity 100% for xylanase enzyme. We found that the glucose has an inhibitory effect on both enzymes activity. Optimum temperature for xylanase is 50^oC, increased temperature decreased productivity of both enzymes. The addition of Ca²⁺, Ba^2+, Mg²⁺ and EDTA strongly enhanced both xylanase activity, While, Co²⁺and Ag⁺ decreased the productivity. On the other hand, the Fe²⁺, Cu²⁺ and Mn²⁺ showed moderate inhibition.

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